Handbook of Vascular Biology Techniques (eBook)
XII, 477 Seiten
Springer Netherland (Verlag)
978-94-017-9716-0 (ISBN)
A wide range of research methods for the study of vascular development, from basic laboratory protocols to advanced technologies used in clinical practice, are covered in this work. A range of methodologies such as molecular imaging platforms and signalling analysis, along with tumour models are collated here.
Four sections explore in vitro techniques, in vivo and ex vivo manipulations, imaging and histological analysis and other novel techniques in vascular biology. Readers will discover basic methodologies used for analysis of endothelial cell growth in vitro, including co-culture models of vessel formation. Authors also explore isolation and purification of cells and methods for analysis of data and visualization of localized vasculature with modern imaging platforms. Both animal models and human disease are covered in this work.
Each chapter contains helpful sections on trouble shooting, additional notes and links, supporting the reader to carry out protocols. This book will appeal to students, researchers and medical professionals working in all vascular-linked fields such as cardio- and cerebrovascular, cancer and dementia.
A wide range of research methods for the study of vascular development, from basic laboratory protocols to advanced technologies used in clinical practice, are covered in this work. A range of methodologies such as molecular imaging platforms and signalling analysis, along with tumour models are collated here.Four sections explore in vitro techniques, in vivo and ex vivo manipulations, imaging and histological analysis and other novel techniques in vascular biology. Readers will discover basic methodologies used for analysis of endothelial cell growth in vitro, including co-culture models of vessel formation. Authors also explore isolation and purification of cells and methods for analysis of data and visualization of localized vasculature with modern imaging platforms. Both animal models and human disease are covered in this work.Each chapter contains helpful sections on trouble shooting, additional notes and links, supporting the reader to carry out protocols. This book will appeal to students, researchers and medical professionals working in all vascular-linked fields such as cardio- and cerebrovascular, cancer and dementia.
PrefaceSection 1 In vitro techniquesChapter 1: In vitro angiogenesis assay - endothelial migration, proliferation, and tube formationChapter 2: Endothelial cell tube formation on basement membrane to study cancer neoangiogenesisChapter 3: Induction of hypoxia in vascular endothelial cell cultureChapter 4: Evaluating In Vitro Angiogenesis using Live Cell ImagingChapter 5: Isolation of Endothelial Progenitor Cells (EPCs)Chapter 6: Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for VasculargenesisChapter 7: Assessment of vascular function and contractility, ex vivo.Chapter 8: Measurement of Aβ uptake by cerebrovascular smooth muscle cellsChapter 9: Measurement of Intracellular Ca2+ in Human Endothelial cells Section 2: In vivo and ex vivo manipulationsChapter 10: Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusionChapter 11: Sponge implant model of inflammatory angiogenesisChapter 12: The chick embryo chorioallantoic membrane assayChapter 13: Dorsal air sac assayChapter 14: Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammalsChapter 15: Hypoxia-induced retinal angiogenesis in adult zebrafishChapter 16: Angiogenesis in regenerating adult zebrafish tail finChapter 17: Methods for studying developmental angiogenesis in zebrafishChapter 18: Isolation and Expansion of Brain Microvascular Endothelial Cells Section 3: Imaging and histological analysisChapter 19: Adipose angiogenesisChapter 20: Assessing tumor angiogenesis in histological samplesChapter 21: Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the embryonic mouse skin and adult mouse cornea.Chapter 22: Computed Tomography Angiography: Fundamental techniques and data interpretationChapter 23: Magnetic Resonance Angiography: Fundamental Techniques and Data InterpretationChapter 24: Single-Photon Emission Tomography of the brain in vascular pathology Section 4: Miscellaneous novel techniques in vascular biologyChapter 25: Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural CuesChapter 26: Preparation of liposomes with dual fluorophores to follow real-time content release in vivo Chapter 27: Vascular Flow Modelling using Computational Fluid DynamicsChapter 28: Reverse Transcription Real-Time PCR Protocol for gene expression analysesChapter 29: Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and HaematopoiesisChapter 30: Genomic microarray analysisChapter 31: Selection of appropriate housekeeping genes for quality controlChapter 32: Endothelial transcriptomic analysisChapter 33: Protocol for Multiplex Amplicon Sequencing using Barcoded PrimersChapter 34: Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells: Identification and QuantificationChapter 35: A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human BloodChapter 36: Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry
| Erscheint lt. Verlag | 27.3.2015 |
|---|---|
| Zusatzinfo | XII, 477 p. 191 illus., 126 illus. in color. |
| Verlagsort | Dordrecht |
| Sprache | englisch |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin |
| Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie | |
| Studium ► 1. Studienabschnitt (Vorklinik) ► Biochemie / Molekularbiologie | |
| Naturwissenschaften ► Biologie | |
| Technik | |
| Schlagworte | angiogenesis • Experimental • Methodology • protocols • Vascular |
| ISBN-10 | 94-017-9716-1 / 9401797161 |
| ISBN-13 | 978-94-017-9716-0 / 9789401797160 |
| Haben Sie eine Frage zum Produkt? |
PDF (Wasserzeichen)Größe: 27,3 MB
DRM: Digitales Wasserzeichen
Dieses eBook enthält ein digitales Wasserzeichen und ist damit für Sie personalisiert. Bei einer missbräuchlichen Weitergabe des eBooks an Dritte ist eine Rückverfolgung an die Quelle möglich.
Dateiformat: PDF (Portable Document Format)
Mit einem festen Seitenlayout eignet sich die PDF besonders für Fachbücher mit Spalten, Tabellen und Abbildungen. Eine PDF kann auf fast allen Geräten angezeigt werden, ist aber für kleine Displays (Smartphone, eReader) nur eingeschränkt geeignet.
Systemvoraussetzungen:
PC/Mac: Mit einem PC oder Mac können Sie dieses eBook lesen. Sie benötigen dafür einen PDF-Viewer - z.B. den Adobe Reader oder Adobe Digital Editions.
eReader: Dieses eBook kann mit (fast) allen eBook-Readern gelesen werden. Mit dem amazon-Kindle ist es aber nicht kompatibel.
Smartphone/Tablet: Egal ob Apple oder Android, dieses eBook können Sie lesen. Sie benötigen dafür einen PDF-Viewer - z.B. die kostenlose Adobe Digital Editions-App.
Buying eBooks from abroad
For tax law reasons we can sell eBooks just within Germany and Switzerland. Regrettably we cannot fulfill eBook-orders from other countries.
aus dem Bereich
