Metastasis Research Protocols
Humana Press Inc. (Verlag)
978-0-89603-615-4 (ISBN)
- Titel ist leider vergriffen;
keine Neuauflage - Artikel merken
Metastasis Research Protocols describes in detail all the methods needed to investigate why and how metastasis occurs. Volume I presents a comprehensive collection of established and leading-edge techniques for analyzing the expression of key molecules and for examining their production at the genetic level. Volume I focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression. The traditional methods range from the histopathological and the immunocytochemical to SDS-PAGE, Western blotting, and enzyme zymography. Newer and more specialized techniques for analyzing the genetic aspects of metastasis include in situ hybridization to localize mRNAs, FISH, CGH, methylation analysis of CpG islands, RT-PCR, differential display. Volume II presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade.
These systems include cell migration assays, assays for matrix degradation enzymes, basement membrane degradation assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models for metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis.
Part I. Selection/Segregation of Cell Populations for In vitro and In Vivo Assays of Metastatic Behavior Cell Separations by Flow Cytometry Derek Davies Immunomagnetic Cell Separation Catherine Clarke and Susan Davies Genetic Modification of Cell Lines to Enhance Their Metastatic Capability Daniel McWilliams and Hilary Collins Part II. In Vitro Assays of Metastatic Behavior Cell Aggregation Assays, Tom Boterberg Marc E. Bracke, Erik A. Bruyneel, and Marc M. Mareel Cell Migration and the Boyden Chamber Nicholas S. Brown and Roy Bicknell Quantification of Cell Motility: Gold Colloidal Phagokinetic Track Assay and Wound Healing Assay Yasufumi Niinaka, Arayo Haga, and Avraham Raz In Vitro Invasion Assay Using Matrigel(R) Debbie M. S. Hall and Susan A. Brooks Membrane Invastion Culture System Mary J. C. Hendrix, Elisabeth A. Seftor, and Richard E. B. Seftor Collagen Invasion Assay Marc E. Bracke, Tom Boterberg, Erik A. Bruyneel, and Marc M. Mareel Chick Heart Invasion Assay Marc E. Bracke, Tom Boterberg, and Marc M. Mareel Adhesion of Tumor Cells to Matrices and Endothelium G. Ed Rainger Assessment of Angiogenic Factors: The Chick Chorioallantoic Membrane Assay Adam Jones, Chisato Fujiyama, Stephen Hague, and Roy Bicknell Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis Nerbil Kilic and Suleyman Ergun Galectin-3 Binding and Metastasis Pratima Nangia-Makker, Yuichiro Honjo, and Avraham Raz Part III. Animal Models of Metastasis Basic Principles for the Study of Metastasis Using Animal Models Suzanne A. Eccles The Chick Embryo Metastasis Model Eric Petitclerc and Peter C. Brooks Gene Transfection for Metastasis Research Using Animal Models Ruth J. Muschel and Jim Hua Theoretical Considerations in Using Animal Models of Metastastis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice Sue A. Watson and Teresa M. Morris Xenograft Models in Immunodeficient Animals: I. Nude Mice: Spontaneous and Experimental Metastatis Models Janet E. Price Xenograft Models in Immunodeficient Animals: II. Use of SCID Mice in Metastasis Research: Breast and Colon Cancer Models of Metastasis Udo Schumacher and Susan A. Brooks Syngeneic Murine Metastasis Model: B16 Melanoma Raffaella Giavazzi and Angela Garofalo Transgenic Animal Models Chantale Guy and Gizela Cordosa Clinically Accurate Orthotopic Mouse Models of Cancer Robert M. Hoffman Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo Marian Rocha, Volker Schirrmacher, and Victor Umansky Green Fluorescent Protein for Metastastis Research Robert M. Hoffman
| Erscheint lt. Verlag | 20.7.2001 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; v. 58 |
| Zusatzinfo | black & white illustrations |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 156 x 234 mm |
| Gewicht | 642 g |
| Einbandart | gebunden |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie |
| Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| ISBN-10 | 0-89603-615-4 / 0896036154 |
| ISBN-13 | 978-0-89603-615-4 / 9780896036154 |
| Zustand | Neuware |
| Haben Sie eine Frage zum Produkt? |
aus dem Bereich
