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MicroRNAs as Tools in Biopharmaceutical Production -

MicroRNAs as Tools in Biopharmaceutical Production

Niall Barron (Herausgeber)

Buch | Softcover
126 Seiten
2014
Springer (Verlag)
978-94-007-9526-6 (ISBN)
CHF 167,95 inkl. MwSt
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This book explores the uses of MicroRNAs in bioprocessing, especially in production of complex proteins in mammalian cells. Covers genomic organization, biogenesis and mode of action; miRNAs as biomarkers; manipulation of expression in cultured cells and more.
Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells,  miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.

Preface.-1 A general introduction to microRNAs, their investigation and exploitation in CHO cell lines.-2 Biogenesis of Mammalian miRNA.-3 Target prediction algorithms and bioinformatics resources for miRNA studies.-4 The CHO miRNA transcriptome.-5 MicroRNAs as engineering targets - pathway manipulation to impact bioprocess phenotypes.-6 MicroRNAs for Enhancement of CHO Cell Proliferation and Stability Insights from Neuroblastoma Studies.-7 MicroRNA Detection Methods for Mammalian Cell Lines and Their Applications in Therapeutic Protein Production.-8 Manipulating Levels of Specific microRNAs in Mammalian Cells.- Index.

Zusatzinfo X, 126 p.
Verlagsort Dordrecht
Sprache englisch
Maße 155 x 235 mm
Themenwelt Medizin / Pharmazie Pflege
Medizin / Pharmazie Pharmazie
Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Zoologie
Naturwissenschaften Chemie Technische Chemie
Technik Umwelttechnik / Biotechnologie
Schlagworte Bioprocessing • Chinese Hamster Ovary cells • microRNA • Recombinant Protein Production
ISBN-10 94-007-9526-2 / 9400795262
ISBN-13 978-94-007-9526-6 / 9789400795266
Zustand Neuware
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