In Situ Hybridization Protocols
Humana Press Inc. (Verlag)
978-1-4939-1458-6 (ISBN)
Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.
Fixation/Permeabilization Procedure for mRNA in situ Hybridization of Zebrafish Whole-mount Oocytes, Embryos, and Larvae.- MicroRNA In Situ Hybridization on Whole Mount Preimplantation Embryos.- Whole-mount in situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies.- Whole Mount in Situ Hybridization using DIG-labeled Probes in Planarian.- In Situ Hybridization on Whole-Mount Zebrafish Embryos and Young Larvae.- LNA-based in Situ Hybridization Detection of mRNAs in Embryos.- Chromogen Detection of microRNA in Frozen Clinical Tissue Samples using LNA™ Probe Technology.- MicroRNA In Situ Hybridisation in Tissue Microarrays.- Fluorescence in situ Hybridization for Detection of Small RNAs on Frozen Tissue Sections.- Sensitive and Specific in situ Hybridisation for Early Drug Discovery.- Zinc Based Fixation for High Sensitivity in situ Hybridization: A Non-Radioactive Colorimetric Method for the Detection of Rare Transcripts on Tissue Sections.- Dual-color Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope.- Fully Automated Fluorescence-based 4-color Multiplex Assay for Co-detection of microRNA and Protein Biomarkers in Clinical Tissue Specimens.- Multiplexed miRNA Fluorescence in situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues.- Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris® RNA Fluorescence in situ Hybridization in Adherent Cells.- Quantitative Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope.- Identification of Low Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia.- In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes.- In situ Hybridization Freeze-assisted Punches (IFAP): Technique for Liquid-based TissueExtraction from Thin Slide-mounted Sections for DNA Methylation Analysis.- miRNA Detection at Single Cell Resolution Using Microfluidic LNA flow-FISH.- PNA-based Fluorescence in situ Hybridization for Identification of Bacteria in Clinical Samples.
Reihe/Serie | Methods in Molecular Biology ; 1211 |
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Zusatzinfo | 52 Illustrations, color; 4 Illustrations, black and white; XI, 275 p. 56 illus., 52 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Schlagworte | Cellular functions • In situ Hybridization • Protein • ribonucleic acid • RNA • RNA molecules • RNA species |
ISBN-10 | 1-4939-1458-8 / 1493914588 |
ISBN-13 | 978-1-4939-1458-6 / 9781493914586 |
Zustand | Neuware |
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