Baculovirus Expression Protocols
Humana Press Inc. (Verlag)
978-0-89603-272-9 (ISBN)
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Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors - leading authorities in the field - present the assorted expression plasmids currently in use, guide the reader through the process of generating and selecting recombinant virus, and describe specific examples of recombinant protein production and purification. The emphasis is on alternative and simpler screening techniques for the selection of recombinant baculovirus. The book also surveys the various insect cell lines currently compatible with the baculovirus system. Highlights include production of recombinant virus using linearized DNA and vectors that contain a b galactosidase indicator and a complete list of expression vectors currently available. Detailed descriptions for the scale-up of protein production using spinner flasks, bioreactors, and insect larvae are also included. Baculovirus Expression Protocols offers both industrial and university-based researchers an outstanding collection of reproducible, step-by-step laboratory protocols.
It will immediately become indispensable for anyone working with baculoviruses and their application in the expression of recombinant proteins in insect cells.
Introduction to the Molecular Biology of Baculoviruses. Baculovirus Transfer Vectors. Insect Cell-Culture Techniques in Serum-Containing Medium. Insect Cell Culture in Serum-Free Medium. Transfection Techniques for Producing Recombinant Baculoviruses. Selection of Recombinant Baculoviruses by Visual Screening. Production of Recombinant Baculoviruses Using Linearized Viral DNA. Rapid Procedures for the Isolation and PCR Analysis of Recombinant Baculovirus. Production of Recombinant Baculoviruses Using Rapid Screening Vectors That Contain the Gene for b-Galactosidase. Fast Isolation of Recombinant Baculovirus by Antibody Screening. Continuous Foreign Gene Expression in Transformed Lepidopteran Insect Cells. Scale-up pf Recombinant Virus and Protein Production in Stirred-Tank Reactors. Expression of Foreign Genes in Bombyx mori Larvae Using Baculovirus Vectors. Expression of Foreign Proteins in Trichoplusia ni Larvae. Use of Baculoviruses as Biological Insecticides. Molecular Approaches to AIDS Vaccine Development Using Baculovirus Expression Vectors. Expression of the Hemagglutinin Protein of Influenza Virus: Analysis of Protein Modifications. Purification of Recombinant Protein Derived from the Baculovirus Expression System Using Glutathione Affinity Agarose. Purification of the Extracellular Domain of the Epidermal Growth Factor Receptor Produced by Recombinant Baculovirus-Infected Insect Cells in a 10-Liter Reactor. Expression and Secretion of a Soluble Form of Myelin-Associated Glycoprotein (MAG). Comparison of Different Cell Lines for the Production of Recombinant Baculovirus Proteins. Development of Lepidopteran Cell Lines. Index.
| Erscheint lt. Verlag | 18.12.2009 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 39 |
| Zusatzinfo | illustrations |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 234 x 156 mm |
| Gewicht | 608 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| ISBN-10 | 0-89603-272-8 / 0896032728 |
| ISBN-13 | 978-0-89603-272-9 / 9780896032729 |
| Zustand | Neuware |
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