Molecular Techniques in Taxonomy
Springer Berlin (Verlag)
978-3-642-83964-1 (ISBN)
Taxonomy is fundamental to understanding the variety of lifeforms, and exciting expansions in molecular biology are re-volutionising the obtained data. This volume reviews the ma-jor molecular biological techniques that are applied in ta-xonomy. The chapters are arranged in three main sections:1)Overviews of important topics in molecular taxonomy; 2) Casestudies of the successful application of molecular methodsto taxonomic and evolutionary questions; 3) Protocols for arange of generally applicable methods. The described techni-ques include DNA-DNA hybridization, DNA fingerprinting, RFLPanalysis, and PCR sequencing.
A Prologue.- Past and Future of Taxonomy.- Overviews.- Taxonomy: an Essential Key to Evolutionary Biology.- Variation at the DNA Level: Something for Everyone.- Molecular Systematics at the Species Boundary: Exploiting Conserved and Variable Regions of the Mitochondrial Genome of Animals via Direct Sequencing from Amplified DNA.- Ribosomal RNA Phylogenies.- Evaluating Gene Versus Genome Evolution.- DNA and Higher Plant Systematics: Some Examples From the Legumes.- DNA-DNA Hybridization: Principles and Results.- Satellite DNA.- DNA Fingerprinting.- The Statistical Interpretation of Hypervariable DNAs.- Case Studies.- A Multidimensional Approach to the Evolution and Systematics of Dolichopoda Cave Crickets.- Sperm and Evolution in Drosophila: Morphological and Molecular Aspects.- Episodic Evolutionary Change in Local Populations.- Searching for Speciation Genes in the Species Pair Drosophila Mojavensis and D. Arizonae.- Colonizing Species of Drosophila.- Molecular Taxonomy in the Control of West African Onchocerciasis.- Protocols.- DNA Protocols for Plants.- Prepartion and Visualization of Mitochondrial DNA for RFLP Analysis.- Hybridization of DNA Probes to Filterbound DNA Using the "DIG-DNA Labeling and Detection System Nonradioactive" (Boehringer Mannheim).- DNA Fingerprinting.- The Polymerase Chain Reaction: DNA Extraction and Amplification.- Protocols for 4-Cutter Blots and PCR Sequencing.- Ribosomal RNA Sequencing.- Quantitative DNA:DNA Hybridization and Hydroxyapatite Elution.- A Protocol for the TEACL Method of DNA-DNA Hybridization.- List of Participants.
| Erscheint lt. Verlag | 15.12.2011 |
|---|---|
| Reihe/Serie | Nato ASI Subseries H: |
| Zusatzinfo | X, 410 p. |
| Verlagsort | Berlin |
| Sprache | englisch |
| Maße | 170 x 242 mm |
| Gewicht | 727 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Botanik |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Naturwissenschaften ► Biologie ► Zoologie | |
| Schlagworte | Biology • DNA • Evolution • evolutionary biology • Gene • Genetics • Genetik • Genetik / Erblehre • molecular aspects • Molecular Biology • Molokularbiologie • PCR • Phylogenie • Plant • plants • plant systematics • Systematics • Systematik • Taxonomie • Taxonomy • Techniken |
| ISBN-10 | 3-642-83964-9 / 3642839649 |
| ISBN-13 | 978-3-642-83964-1 / 9783642839641 |
| Zustand | Neuware |
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