Proteome and Protein Analysis
Springer Berlin (Verlag)
978-3-642-64060-5 (ISBN)
Topics included are: Protein sequencing analysis, protein and peptide sample preparation, mass spectrometry, NMR, analysis of post-translational modifications, purification of recombinant proteins, protein-protein and protein-DNA interactions, structure prediction, modeling and protein folding, functional implications of protein domains and newly emerging methods for the investigation of the proteome, allowing to analyse the expression of genes
I Proteomics and Mass Spectrometry.- 1 Strategies and Methods for Proteome Analysis.- 2 Protein Sequencing or Genome Sequencing. Where Does Mass Spectrometry Fit into the Picture?.- 3 Reversible Negative Staining of Protein on Electrophoresis Gels by Imidazole-Zinc Salts: Micropreparative Applications to Proteome Analysis by Mass Spectrometry.- 4 Direct Analysis of Protein Complexes.- 5 De novo Sequencing of Proteins With Mass Spectrometry Using the Differential Scanning Technique.- 6 Characterization Of Gel Separated Proteins.- II Structure Analysis.- 7 Protein Structure and Dynamics by NMR in Solution.- 8 Elucidation of Functionally Significant Structural Modifications by Matrix-Assisted Laser Deorption/Ionization Time-of-Flight Mass Spectrometry with Post-Source Decay Analysis.- 9 Detection of Specific Zinc Finger Peptide Complexes with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.- 10 Epitode Mapping for the Monoclonal Antibody that Inhibits Intramolecular Flavin to Heme Electron Transfer in Flavocytochrome B2 from Baker's Yeast (L-Lactate Dehydrogenase).- 11 A MALDI-TOF Mass Spectrometry Approach to Investigate the Defense Reactions in Drosophila Melanogaster, an Insect Model for the Study of Innate Immunity.- 12 A New Method for the Isolation of the C-Terminal Peptide of Proteins by the Combination of Selective Blocking of Proteolytic Peptides and Cation Exchange Chromatography.- 13 The Folding Pathway of Disulfide Containing Proteins.- 14 Cavity Supported HPLC of cis/trans Isomers of Proline Containing Peptides Using Cyclodextrins and Calixarenes.- 15 The Perception of Hydrophobic Clusters in the Native and Partially Unfolded States of a Protein.- 16 Structure, Dynamics and Function of the Proton Pump Bacteriorhodopsin.- 17 StructuralCharacterization of Porcine Seminal Plasma Psp-I/Psp-li, A Paradigm Spermadhesin.- 18 Expression and Characterization of Saposin-Like-Proteins.- III Protein-Protein and Protein-DNA Interaction.- 19 A Structural Model for the P53 Complex with DNA Response Elements: Implications for P53 Function and Future Research Directions.- 20 Overproduction, Purification and Structural Studies on the Zn Containing S14 Ribosomal Protein from Thermus thermophilus.- 21 Bimolecular Interaction of Matrix Metalloproteinases and Their Inhibitors TIMPs.- IV Posttranslational Modifications.- 22 Enzymatic and Chemical Deblocking of N-Terminally Modified Proteins.- 23 Exploring Functions of Glycosylation in Host Defence Using Novel Oligosaccharide Sequencing Technology.- 24 Proteoglycans: Biological Roles and Strategies for Isolation and Determination of their Glycan Constituents.
| Erscheint lt. Verlag | 9.2.2012 |
|---|---|
| Zusatzinfo | XI, 372 p. |
| Verlagsort | Berlin |
| Sprache | englisch |
| Maße | 155 x 235 mm |
| Gewicht | 586 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Schlagworte | interaction • Massenspektrometrie • Posttranslational modification • Protein-DNA Interaction • Protein-Protein • proteins • Proteinsequenzierung • protein structure analysis • Proteinstrukturanalyse • Proteomeanalyse • Proteomics • Translation |
| ISBN-10 | 3-642-64060-5 / 3642640605 |
| ISBN-13 | 978-3-642-64060-5 / 9783642640605 |
| Zustand | Neuware |
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