Flow Cytometry (eBook)

Principles and Applications

Marion G. Macey (Herausgeber)

eBook Download: PDF

2007 | 2007
X, 294 Seiten
Humana Press (Verlag)
978-1-59745-451-3 (ISBN)

Infos

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers.

The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

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Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.

Inhalt

Preface 5
Contents 6
Contributors 7
1 Principles of Flow Cytometry 8
2 Cell Preparation 23
3 Fluorochromes and Fluorescence 65
4 Quality Control in Flow Cytometry 119
5 Experimental Design, Data Analysis, and Fluorescence Quantitation 138
6 Apoptosis Detection by Flow Cytometry 151
7 DNA Analysis by Flow Cytometry 168
8 Immunological Studies of Human Cells 183
9 Calcium: Cytoplasmic, Mitochondrial, Endoplasmic Reticulum, and Flux Measurements 210
10 Further Functional Studies 219
11 Cell Sorting by Flow Cytometry 257
Appendix: Useful Internet Sites 277
Index 286

Produkt-Details

• Erscheint lt. Verlag: 3.11.2007
• Zusatzinfo: X, 294 p. 72 illus.
• Verlagsort: Totowa
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin
• Themenwelt: Medizin / Pharmazie ► Studium
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Schlagworte: DNA • Laboratory • membrane potential • Oxidative Metabolism • proteins
• ISBN-10: 1-59745-451-6 / 1597454516
• ISBN-13: 978-1-59745-451-3 / 9781597454513