Apoptosis and Cancer

Methods and Protocols

Buch | Softcover

266 Seiten

2010 | Softcover reprint of hardcover 1st ed. 2008
Humana Press Inc. (Verlag)
978-1-61737-582-8 (ISBN)

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Our understanding of the apoptotic pathway and its regulation has improved significantly in the last 10 years. However, as our knowledge of the intricate intracellular events increase, we realize the complexity of the regulatory mechanisms that control it. As such, Apoptosis and Cancer describes the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. This book is a collaboration between academics- and industry-based scientists. The chapters have the technical development characteristic of an academic environment and the standardized system necessary for translating a product to general use, as provided by the industry. Thus, the protocols described within this groundbreaking new volume will aid both an academic laboratory interested in further characterizing the mechanism of apoptosis and an industry laboratory interested on identifying new target molecules or screening for new compounds with potential clinical use. The authors – all leaders in the field – have created a comprehensive text that covers the newest available techniques as well as conventional basic techniques. Apoptosis and Cancer will undoubtedly provide the necessary resources to everyone interested in the area of apoptosis.

Preface.- Contributors.- Modulation of Apoptosis to Reverse ChemoresistanceGil Mor, Michele K. Montagna, and Ayesha B. Alvero.- Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-Induced ApoptosisSuparna Mazumder, Dragos Plesca, and Alexandru Almasan.- Flow Cytometry Enumeration of Apoptotic Cancer Cells by Apoptotic RateDavid Diaz, Alfredo Prieto, Eduardo Reyes, Hugo Barcenilla, Jorge Monserrat, and Melchor Alvarez-Mon.- Detection of Cancer-Related Proteins in Fresh-Frozen Ovarian Cancer Samples Using Laser Capture MicrodissectionDan-Arin Silasi, Ayesha B. Alvero, Jechiel Mor, Rui Chen, Han-Hsuan Fu, Michele K. Montagna, and Gil Mor.- Flow Cytometric Detection of Activated Caspase-3Richard Fox and Martine Aubert.- The ChemoFx™ Assay: An Ex Vivo Chemosensitivity and Resistance Assay for Predicting Patient Response to Cancer ChemotherapyStacey L. Brower, Jeffrey E. Fensterer, and Jason E. Bush.- Correlation of Caspase Activity and In Vitro Chemo-Response in Epithelial Ovarian Cancer Cell LinesAyesha B. Alvero, Michele K. Montagna, and Gil Mor.- Assessing Expression of Apoptotic Markers Using Large Cohort Tissue MicroarraysElah Pick, Mary M. McCarthy, and Harriet M. Kluger.- Mitochondria Potential, Bax “Activation,” and Programmed Cell DeathC. Michael Knudson and Nicholas M. Brown.- In Vitro and In Vivo Apoptosis Detection Using Membrane Permeant Fluorescent-Labeled Inhibitors of CaspasesBrian W. Lee, Michael R. Olin, Gary L. Johnson, and Robert J. Griffin.- Caspase Activity AssaysAndrew L. Niles, Richard A. Moravec, and Terry L. Riss.- Multiplex Caspase Activity and Cytotoxicity AssaysAndrew L. Niles, Richard A. Moravec, and Terry L. Riss.- Homogeneous, Bioluminescent Proteasome AssaysMartha A. O’Brien, Richard A. Moravec, Terry L. Riss, andRobert F. Bulleit.- A Simple Method for Profiling miRNA ExpressionJia-Wang Wang and Jin Q. Cheng.- Apoptotic Caspase Activation and ActivityJean-Bernard Denault and Guy S. Salvesen.- Biochemical Analysis of the Native TRAIL Death-Inducing Signaling ComplexHenning Walczak and Tobias L. Haas.- Laser Microdissection Sample Preparation for RNA AnalysesChristopher J. Vega.

Erscheint lt. Verlag	19.11.2010
Reihe/Serie	Methods in Molecular Biology ; 414
Zusatzinfo	69 Illustrations, black and white; XI, 266 p. 69 illus.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
	Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-61737-582-9 / 1617375829
ISBN-13	978-1-61737-582-8 / 9781617375828
Zustand	Neuware