DNA Repair Protocols

Prokaryotic Systems

Buch | Softcover

192 Seiten

2010 | Softcover reprint of hardcover 1st ed. 2000
Humana Press Inc. (Verlag)
978-1-61737-112-7 (ISBN)

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When setting out to decide on the content of DNA Repair Protocols: Prokaryotic Systems, I was conscious of the need to portray the vast array of pathways and enzymatic activities that are part of the discipline of DNA repair. In addition to the classical DNA repair activities, I wanted to convey the significant interest that has been generated in recent years in the use of the proteins and repair systems as research tools, much like the use of restriction enzymes over the last few decades. Therefore, in addition to chapters deta- ing protocols for investigating specific repair activities, I have included s- eral chapters in this book on the applied use of DNA repair proteins and systems. The many years of research on bacterial DNA repair systems have allowed us to really understand the majority of DNA repair pathways in bac- rial cells. Building on this knowledge, research has lead to major advances in understanding mammalian DNA repair and uncovered its links to human d- ease, such as DNA mismatch repair and colon cancer, nucleotide excision repair and xeroderma pigmentosum, DNA helicase function in Bloom’s s- drome, and so on. Such have been the advances that Science magazine iden- fied the collective DNA repair systems as its “Molecule of the Year” in 1994.

Assays To Detect And Quantitate DNA Repair Activity.- Repair of A/G and A/8-oxoG Mismatches by MutY Adenine DNA Glycosylase.- Assays for the Repair of Oxidative Damage by Formamidopyrimidine Glycosylase (Fpg) and 8-)Oxoguanine DNA Glycosylase (OGG-1).- Detection and Quantitation of Uracil DNA Glycosylase Activity.- Detection of DNA Deoxyribophosphodiesterase Activity.- O 6-Alkylguanine-DNA Alkytransferase Assay.- The E. coli Vsr Endonuclease.- Coupling of DNA Helicase Function to DNA Strand Exchange Activity.- Detection and Quantitation of RecBCD Enzyme (Exonuclease V) Activity.- Holliday Junction Branch Migration and Resolution.- MutS–DNA Interactions and DNase Protection Analysis with Surface Plasmon Resonance.- Use Of DNA Repair Enzymes As Tools In Molecular Biology.- Flexible Genetic Engineering Using RecA Protein.- Interspecies Recombination and Mismatch Repair.- The Use of Immobilized Mismatch Binding Protein in Mutation/SNP Detection.- Use of Uracil DNA Glycosylase in the Detection of Known DNA Mutations and Polymorphisms.- Use of Uracil DNA Glycosylase in Scanning for Unknown DNA Mutations and Polymorphisms.- The Use of Resolvases T4 Endonuclease VII and T7 Endonuclease I in Mutation Detection.

Erscheint lt. Verlag	10.11.2010
Reihe/Serie	Methods in Molecular Biology ; 152
Zusatzinfo	X, 192 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-61737-112-2 / 1617371122
ISBN-13	978-1-61737-112-7 / 9781617371127
Zustand	Neuware