Peptide Synthesis

Methods and Protocols

Waleed M. Hussein, Mariusz Skwarczynski, Istvan Toth (Herausgeber)

Buch | Softcover

338 Seiten

2020 | 1st ed. 2020
Springer-Verlag New York Inc.
978-1-0716-0229-4 (ISBN)

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This book provides a variety of procedures for synthetically producing peptides and their derivatives, ensuring the kind of precision that is of paramount importance for successful synthesis. Numerous techniques relevant to drugs and vaccines are explored, such as conjugation and condensation methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Peptide Synthesis: Methods and Protocols serves as an essential guide to the many crucial processes that will allow researchers to efficiently prepare, purify, characterize, and use peptides for chemical, biochemical, and biological studies.

Solution Phase Peptide Synthesis: The Case of Biphalin.- Application of Fmoc-SPPS, Thiol-Maleimide Conjugation and Copper(I)-Catalyzed Alkyne-Azide Cycloaddition “Click” Reaction in the Synthesis of a Complex Peptide-Based Vaccine Candidate against Group A Streptococcus.- In Situ Neutralization Protocols for Boc-SPPS.- Anhydrous Hydrogen Fluoride Cleavage in Boc Solid Phase Peptide Synthesis.- Automated Solid-Phase Peptide Synthesis.- New Developments in Microwave-Assisted Solid Phase Peptide Synthesis.- Protecting Groups in Peptide Synthesis.- Synthesis of O-Acyl Isopeptides: Stepwise and Convergent Solid-Phase Synthesis.- An Isodipeptide Building Block for Microwave-Assisted Solid-Phase Synthesis of Difficult Sequence-Containing Peptides.- SPOT Synthesis: The Solid-Phase Peptide Synthesis on Planar Surfaces.- Solid-Phase Synthesis of Wollamide Cyclohexapeptide Analogs.- Synthesis of Branched Peptides via a Side-Chain Benzyl Ester.- Solid Phase Synthesis of Octapeptin Lipopeptides.- Peptidomimetic Synthesis―Drug Discovery for Alzheimer’s Disease.- Synthesis of Amide Backbone-Modified Peptides.- Synthesis of NickFects, a New Family of CPPs, by Solid-Phase Peptide Synthesis.- Chemoenzymatic Synthesis of HIV-1 Glycopeptide Antigens.- Synthesis of Antimicrobial Lipopeptides Using the “CLipPA” Thiol-Ene Reaction.- Decarboxylative Couplings for Late-Stage Peptide Modifications.- Peptide and Pseudopeptide Bond Synthesis in Phosphorus Dipeptide Analogs.- Micro-Bioreactor Techniques for the Production and Spectroscopic Characterization of Microbial Peptides.- Metal Peptide Complexes for Study Neurodegenerative Diseases.

Erscheinungsdatum	16.01.2021
Reihe/Serie	Methods in Molecular Biology ; 2103
Zusatzinfo	56 Illustrations, color; 79 Illustrations, black and white; XI, 338 p. 135 illus., 56 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Pharmazie
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Chemie ► Organische Chemie
	Naturwissenschaften ► Chemie ► Technische Chemie
	Technik ► Maschinenbau
Schlagworte	Biosynthetic methods • Conjugation • Drug-like properties • Peptide derivatives • Product yield and purity • Vaccine antigens
ISBN-10	1-0716-0229-2 / 1071602292
ISBN-13	978-1-0716-0229-4 / 9781071602294
Zustand	Neuware