Advances in Single Molecule, Real-Time (SMRT) Sequencing

Adam Ameur, Matthew S. Hestand (Herausgeber)

Buch | Softcover

128 Seiten

2019
MDPI (Verlag)
978-3-03921-700-7 (ISBN)

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PacBio’s single-molecule real-time (SMRT) sequencing technology offers important advantages over the short-read DNA sequencing technologies that currently dominate the market. This includes exceptionally long read lengths (20 kb or more), unparalleled consensus accuracy, and the ability to sequence native, non-amplified DNA molecules. From fungi to insects to humans, long reads are now used to create highly accurate reference genomes by de novo assembly of genomic DNA and to obtain a comprehensive view of transcriptomes through the sequencing of full-length cDNAs. Besides reducing biases, sequencing native DNA also permits the direct measurement of DNA base modifications. Therefore, SMRT sequencing has become an attractive technology in many fields, such as agriculture, basic science, and medical research. The boundaries of SMRT sequencing are continuously being pushed by developments in bioinformatics and sample preparation. This book contains a collection of articles showcasing the latest developments and the breadth of applications enabled by SMRT sequencing technology.

Erscheinungsdatum	24.12.2019
Verlagsort	Basel
Sprache	englisch
Maße	170 x 244 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	allele-specific analysis • bone marrow cell subpopulations • Cladobotryum protrusum • cobweb disease • cytochrome P450 enzyme (CYP) • de novo assembly • de novo genome assembly • DNA Methylation • full length RNAseq • gene expression • Gloeostereum incarnatum • GRCh38 • human reference genome • human whole-genome sequencing • insect genome • long-read SMRT sequencing • low-input DNA • Mosquito • mRNA isoforms • mycoparasite • N/A • Next generation sequencing • Optical Mapping • PacBio • PacBio single molecule real-time sequencing • population sequencing • protein isoforms • secondary metabolite • single molecule real-time sequencing • SMRT sequencing • Statistical Methods • Swedish population • terpenoid • Tricoplusia ni • whole genome sequencing
ISBN-10	3-03921-700-3 / 3039217003
ISBN-13	978-3-03921-700-7 / 9783039217007
Zustand	Neuware