RNAi and microRNA-Mediated Gene Regulation in Stem Cells
Humana Press Inc. (Verlag)
978-1-4939-5749-1 (ISBN)
Authoritative and cutting-edge, RNAi and microRNA-Mediated Gene Regulation in Stem Cells: Methods, Protocols, and Applications serves as a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and the exciting areas of medicine, including regenerative medicine, aging, cancer, and neurological disorders, that can be advanced through this expanding area of research.
RNAi in Stem Cells: Current Status and Future Perspectives.- A Brief Introduction to RNAi and MicroRNAs in Stem Cells.- RNA Interference.- Forward RNAi Screens in Human Stem Cells.- A Recessive Genetic Screen for Components of the RNA Interference Pathway in Mouse Embryonic Stem Cells.- Construction and Application of Random dsRNA Interference Library for Functional Genetic Screens in Embryonic Stem Cells.- Establishing Efficient siRNA Knockdown in Stem Cells Using Fluorescent Oligonucleotides.- Loss-of-Function Studies in Mouse Embryonic Stem Cells Using the pHYPER shRNA Plasmid Vector.- Regulation and/or Repression of Cholinergic Differentiation of Murine Embryonic Stem Cells Using RNAi Directed Against Transcription Factor L3/Lhx8.- Silencing of Rho-GDI? by RNAi Promotes the Differentiation of Neural Stem Cells.- RNAi Knockdown of Redox Signaling Protein Ape1 in the Differentiation of Mouse Embryonic Stem Cells.- Proteins Involved in Cell Migration from Glioblastoma Neurospheres Analyzed by Overexpression and siRNA-Mediated Knock-Down.- An Efficient Transfection Method for Mouse Embryonic Stem Cells.- Semi-quantitative Analysis of Transient Single-Cell Gene Expression in Embryonic Stem Cells by Femtoinjection.- MicroRNAs.- Preparation and Analysis of MicroRNA Libraries Using the Illumina Massively Parallel Sequencing Technology.- Assessing In Vivo MicroRNA Function in the Germline Stem Cells of the Drosophila Ovary.- Monitoring MicroRNA Expression During Embryonic Stem-Cell Differentiation Using Quantitative Real-Time PCR (qRT-PCR).- Engineering Human Mesenchymal Stem Cells to Release Adenosine Using miRNA Technology.- Efficient Gene Knockdowns in Mouse Embryonic Stem Cells Using MicroRNA-Based shRNAs.
Erscheinungsdatum | 19.08.2017 |
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Reihe/Serie | Methods in Molecular Biology ; 650 |
Zusatzinfo | XI, 261 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik |
Naturwissenschaften ► Biologie ► Allgemeines / Lexika | |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 1-4939-5749-X / 149395749X |
ISBN-13 | 978-1-4939-5749-1 / 9781493957491 |
Zustand | Neuware |
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