The Skin and Gene Therapy

Ulrich R. Hengge, Beatrix Volc-Platzer (Herausgeber)

Buch | Softcover

XIX, 301 Seiten

2012 | 1. Softcover reprint of the original 1st ed. 2001
Springer Berlin (Verlag)
978-3-642-63093-4 (ISBN)

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Gene therapy is a novel concept of treating diseases at the molec ular level by rescuing genetic defects through the introduction of corrective genes. The knowledge of genetic defects underlying var ious diseases is being rapidly accumulated, and new genotypic findings are being reported with striking frequency. The elucida tion of the genetic background of many diseases, including hered itary blistering and hyperkeratotic skin disorders, has provided the necessary knowledge to correct inborn genetic aberrations in ani mal models and patient-derived tissue. However,skin is not only a target organ for gene therapy ofcutaneous disorders. Dueto its bio logical properties, easy accessibility, and convenient, established culture conditions and grafting techniques, skin has rapidly be come an important target for correcting other diseases as well. Both skin fibroblasts and epidermal keratinocytes have been successfully transfected ex vivo and in vivo using viral and nonvir al gene transfer methods. Notably,in the first corrective gene ther apy approach in 1987, fibroblasts from adenosine-deaminase (ADA)-deficienthumans with severe combined immunodeficiency (SCID) syndrome were transduced using retroviral vectors. More over, skin cells are capable of synthesizing various proteins that are therapeutic, either locally or systemically. Human epidermal grafts expressing human growth hormone after retroviral gene transfer and grafting onto athymic mice released human growth hormone into the bloodstream of the transplanted mice, for a lim ited time.

Basic Aspects.- 1 The Epidermal Barrier and Strategies for Surmounting It: An Overview.- 2 Stem Cells, Differentiation and Renewal Kinetics of Keratinocytes: Implications for Cutaneous Gene Therapy.- 3 Relevant Animal Models for Skin Gene Therapy.- 4 Nonviral Gene Transfer into the Skin.- 5 Safety and Pharmacokinetics of Naked Plasmid DNA: Studies on Dissemination and Ectopic Expression.- 6 Uptake of DNA by Keratinocytes.- Treatment of Skin Diseases.- Gene Therapy of Inherited Skin Diseases.- Gene Transfer Strategies in Tissue Repair.- Systemic Effects of Skin Gene Therapy.- The Use of Skin-Directed Gene Therapy in the Treatment of Systemic Diseases.- Keratinocyte Gene Therapy Using Cytokine Genes.- Genetic Vaccination Using the Skin.- Principles of Genetic Immunization.- Systematic Modulation of Immune Responses by CpG DNA.- Genetic and Dendritic Cell Vaccination as a Novel Therapy for Melanoma.- Molecular Strategies Interfering with Tumor Progression of Melanoma and Improving Anti-Tumor Immunity.- Prophylactic and Therapeutic DNA Vaccines Against Infectious Diseases.

Erscheint lt. Verlag	23.10.2012
Zusatzinfo	XIX, 301 p. 39 illus., 8 illus. in color.
Verlagsort	Berlin
Sprache	englisch
Maße	155 x 235 mm
Gewicht	492 g
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Dermatologie
Schlagworte	Biosafety • Bio-Sicherheit • Cell • Cytokine • Dermatologie • Dermatologie / Hautkrankheiten • Dermatology • Diseases • DNA • gene therapy • genetic immunization • genetische Immunisierung • gene transfer • Gentherapie • Gentransfer • infectious disease • melanoma • Stem Cells • therapy • tissue • Tumor • tumor progression • Vaccine
ISBN-10	3-642-63093-6 / 3642630936
ISBN-13	978-3-642-63093-4 / 9783642630934
Zustand	Neuware