Nitric Oxide, Part D (eBook)

Enrique Cadenas, Lester Packer (Herausgeber)

eBook Download: PDF

2002 | 1. Auflage
514 Seiten
Elsevier Science (Verlag)
978-0-08-049703-7 (ISBN)

Infos

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. - Presents information on nitrosothiols and nitric oxide in cell signaling - Details nitric oxide and mitochondrial functions - Explains nitric oxide synthases

Inhalt

Cover 1
Table of Contents 6
Contributors to Volume 359 10
Preface 16
Volume in Series 18
Section I: Detection of Nitric Oxide 40
Chapter 1. Electron Paramagnetic Resonance for Quantitation of Nitric Oxide in Aqueous Solutions 42
Chapter 2. In Vitro Detection of Nitric Oxide and Nitroxyl by Electron Paramagnetic Resonance 57
Chapter 3. Iron Dithiocarbamate as Spin Trap for Nitric Oxide Detection: Pitfalls and Successes 66
Chapter 4. Advanced Spin Trapping of Vascular Nitric Oxide Using Colloid Iron Diethyldithiocarbamate 81
Chapter 5. Simultaneous Detection of pO2 and NO by Electron Paramagnetic Resonance 91
Chapter 6. Electron Paramagnetic Resonance Studies Nitric Oxide in Living Mice 105
Chapter 7. Measuring End Products of Nitric Oxide in Vivo 114
Chapter 8. Guide for the Use of Nitric Oxide (NO) Donors as Probes of the Chemistry of NO and Related Redox Species in Biological Systems 123
Chapter 9. Amperometric Measurement of Nitric Oxide Using a Polydemethylsiloxane-Coated Electrode 144
Chapter 10. Nitric Oxide Monitoring in Hippocampal Brain Slices Using Electrochemical Methods 150
Chapter 11. Electrochemical Detection of Physiological Nitric Oxide: Materials and Methods 164
Chapter 12. Detection of Nitric Oxide Production by Fluorescent Indicators 173
Chapter 13. Specific Analysis of Nitrate and Nitrite by Gas Chromatography/Mass Spectrometry 187
Chapter 14. Griess Method for Nitrite Measurement of Aqueous and Protein-Containing Samples 197
Chapter 15. Gas-Phase Oxidation and Disproportionation of Nitric Oxide 207
Chapter 16. Detection and Quantification of Nitric Oxide (NO) Synthase-Independent Generation of NO 219
Chapter 17. Nitric Oxide-Dependent Vasodilation in Human Subjects 225
Chapter 18. Antioxidant and Diffusion Properties of Nitric Oxide in Low-Density Lipoprotein 239
Chapter 19. Measurements of Redox Control of Nitric Oxide Bioavailability 248
Section II: Nitrosothiols and Nitric Oxide in Cell Signaling 256
Chapter 20. Reactions of S-Nitrosothiols with L-Ascorbic Acid in Aqueous Solution 258
Chapter 21. Nitrosothiols and S-Nitrosohemoglobin 268
Chapter 22. Nitrosothiol Processing by Platelets 277
Chapter 23. Nitric Oxide-Activated Glutathione Sepharose 284
Chapter 24. Measurement of Protein Nitration and S-Nitrosothiol Formation in Biology and Medicine 295
Chapter 25. S-Glutathionylation of NF-.B Subunit p50 307
Chapter 26. Intercellular Signaling Mediated by Nitric Oxide in Human Glioblastoma Cells 319
Chapter 27. L-Arginine Metabolism in Trypanosoma cruzi in the Regulation of Programmed Cell Death 325
Section III: Nitric Oxide and Mitochondrial Functions 342
Chapter 28. Measurement of Mitochondrial Respiratory Thresholds and the Control of Respiration by Nitric Oxide 344
Chapter 29. Determination of Nitric Oxide-Induced Effects on Tissue Levels of Glutathione and Mitochondrial Membrane Potential 358
Chapter 30. Pharmacological Regulation of Mitochondrial Nitric Oxide Synthase 367
Chapter 31. Characterization of Mitochondrial Nitric Oxide Synthase 378
Section IV: Peroxynitrite 390
Chapter 32. Peroxynitrite Formation from Biochemical and Cellular Fluxes of Nitric Oxide and Superoxide 392
Chapter 33. Evaluation of Antioxidant Activity Using Peroxynitrite as a Source of Radicals 405
Chapter 34. Peroxynitrite Reactions with Heme and Heme- Thiolate (P450) Proteins 418
Chapter 35. Gas Chromatography/Mass Spectrometry Assay for 3-Nitrotyrosine 429
Chapter 36. Quantitation and Localization of Tyrosine Nitration in Proteins 438
Section V: Nitric Oxide Synthases 450
Chapter 37. Neuronal Nitric Oxide Synthases in Brain and Extraneural Tissues 452
Chapter 38. Visualization and Distribution of Neuronal Nitric Oxide Synthase-Containing Neurons 463
Chapter 39. Quantitative Measurement of Endothelial Constitutive Nitric Oxide Synthase 472
Chapter 40. Quantitation of Expressed Message for Inducible Nitric Oxide Synthase 484
Chapter 41. Inducible Nitric Oxide Synthase Knockout Mouse and Low-Density Lipoprotein Oxidation 491
Chapter 42. Nitric Oxide and Resolution of Inflammation 498
Author Index 506
Subject Index 540

Produkt-Details

• Erscheint lt. Verlag: 9.12.2002
• Sprache: englisch
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
• Themenwelt: Technik
• ISBN-10: 0-08-049703-9 / 0080497039
• ISBN-13: 978-0-08-049703-7 / 9780080497037