Vision Research Protocols

Buch | Softcover

320 Seiten

2011 | Softcover reprint of hardcover 1st ed. 2001
Humana Press Inc. (Verlag)
978-1-61737-214-8 (ISBN)

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Elizabeth Rakcozy and a team of leading clinical and experimental scientists describe in step-by-step detail the key techniques essential to effective molecular biological research in ophthalmology and optometry. These readily reproducible methods are adapted to the special requirements of vision research, with coverage that ranges from the most basic to the most sophisticated technologies. Included are methods for the down-regulation of gene expression, new gene therapy techniques, and for the development of transgenic and knockout animal models for testing novel therapies. Eminently accessible and clinically relevant, Vision Research Protocols provides experimental and biomedical investigators in ophthalmology and optometry with a rich panoply of most powerful tools with which to ask--and answer--all the important questions emerging from the dramatically advancing work in vision research today.

Basic Molecular Biology Techniques.- Transient Transfection of Human Retinoblastoma Cells.- In Situ Hybridization.- Immunocytochemical Studies of the Retina.- Antisense DNA Technology.- Inhibition of Gene Expression by Ribozymes.- Intraocular Delivery of Recombinant Virus.- Preparation of Recombinant Adenoviruses.- Generation of Recombinant Adeno-Associated Virus.- Preparation of Recombinant Retroviruses.- Generation of Transgenic Mice for Studies of Ocular Development and Disease.- Generation of Knockout Animal Models.- Mutation Analysis of Ocular Genes.- Ophthalmologists’ Perspective.

"...a highly detailed book on experimental techniques in vision research...highly recommended book for everyone with an interest in molecular biology and eye research." -Acta Ophthalmologica Scandinavica

Reihe/Serie	Methods in Molecular Medicine ; 47
Zusatzinfo	X, 320 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Augenheilkunde
ISBN-10	1-61737-214-5 / 1617372145
ISBN-13	978-1-61737-214-8 / 9781617372148
Zustand	Neuware