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Plant Metabolism

Methods and Protocols

Ganesh Sriram (Herausgeber)

Buch | Hardcover

318 Seiten

2013
Humana Press Inc. (Verlag)
978-1-62703-660-3 (ISBN)

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In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.

MeRy-B, a Metabolomic Database and Knowledge Base for Exploring Plant Primary Metabolism.- Targeted Deuteration of Polyphenolics for their Qualitative and Quantitative Metabolomic Analysis in Plant-Derived Extracts.- Relative Quantitation in Single Cell Metabolomics by Laser Ablation Electrospray Mass Spectrometry.- Quantification of Plant Volatiles.- Quantitative Imaging Approaches for Small Molecule Measurements using FRET Sensors in Plants.- Isotopomer Measurement Techniques in Metabolic Flux Analysis I: Nuclear Magnetic Resonance.- Isotopomer Measurement Techniques in Metabolic Flux Analysis II: Mass Spectrometry.- Mathematical Modeling of Isotope Labeling Experiments for Metabolic Flux Analysis.- Optimal Design of Isotope Labeling Experiments.- Putting the Plant Metabolic Network Pathway Databases to Work: Going Offline to Gain New Capabilities.- Elucidation of Metabolic Pathways from Enzyme Classification Data.- Deducing Intracellular Distributions of Metabolic Pathways from Genomic Data.- Genome-scale Models of Plant Metabolism.- Elementary Flux Modes, Flux Balance Analysis and their Application to Plant Metabolism

Junker.- Systems Approaches to Unraveling Plant Metabolism: Identifying Biosynthetic Genes of Secondary Metabolic Pathways.- Applications of Kinetic Modeling to Plant Metabolism.- Kinetic Modeling of Plant Metabolism and its Predictive Power – Peppermint Essential Oil Biosynthesis as an Example.

Erscheint lt. Verlag	12.11.2013
Reihe/Serie	Methods in Molecular Biology ; 1083
Zusatzinfo	43 Illustrations, color; 36 Illustrations, black and white; X, 318 p. 79 illus., 43 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Informatik ► Weitere Themen ► Bioinformatik
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Botanik
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Bioinformatic tools • biosynthetic genes • elementary flux mode analysis • enzyme classification data • Fluxomics • genome-scale metabolic modeling • Genomic data • interpret isotope labeling • kinetic modeling • metabolic pathways and networks • Metabolomics • metabolomic techniques
ISBN-10	1-62703-660-1 / 1627036601
ISBN-13	978-1-62703-660-3 / 9781627036603
Zustand	Neuware